Table 4.
Difference in predicted and experimental ΔG°37 values for d(ATGCGCAT)2 in the presence of small molecular weight crowders
| Crowding Agent | Experimental ΔG°37 (kcal/mol) | 20% PEG 200 RNA NN ΔG°37 (kcal/mol) | PEG ΔΔG°37a (kcal/mol) | Standard RNA NN ΔG°37 (kcal/mol) | RNA ΔΔG°37b (kcal/mol) | Standard DNA NN ΔG°37 (kcal/mol) | DNA ΔΔG°37c (kcal/mol) |
|---|---|---|---|---|---|---|---|
| 20% EG | -9.30 | -8.74 | 0.56 | -10.20 | -0.90 | -8.65 | 0.65 |
| 20% PEG 200 | -8.70 | -8.74 | -0.04 | -10.20 | -1.50 | -8.65 | 0.05 |
| 20% PEG 1000 | -9.90 | -8.74 | 1.16 | -10.20 | -0.30 | -8.65 | 1.25 |
| Averaged | 0.59 | 0.90 | 0.65 |
aDifference between experimental ΔG°37 and ΔG°37 predicted using 20% PEG 200 RNA nearest neighbor parameters.
bDifference between experimental ΔG°37 and ΔG°37 predicted using standard RNA nearest neighbor parameters.
cDifference between experimental ΔG°37 and ΔG°37 predicted using standard DNA nearest neighbor parameters.
dAverage of the absolute value of each ΔG°37 value.