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. 2019 Jan 18;47(7):3485–3502. doi: 10.1093/nar/gkz025

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Transcription inhibition alleviates replication fork progression defect in WRN-deficient cells. (A) Experimental scheme of dual labelling of DNA fibers in Werner syndrome (WS) and WS-corrected (WSWRN) cells. Cells were pre-treated with DRB, then pulse-labelled with CldU and treated or not with Aph. After washing, cells were pulse-labelled with IdU as indicated. Representative DNA fiber images are shown. (B) Graph shows the analysis of replication fork velocity (fork speed) in the cells. The length of the green tracks were measured. Mean values are represented as horizontal black lines. (ns, not significant; ****P < 0.0001; two-tailed Student's t test). (C) Graph shows the evaluation of fork symmetry in the cells as reported in the scheme. Mean values are represented as horizontal black lines. (ns, not significant; ***P < 0.001; ****P < 0.0001; two-tailed Student's t test).