miR-375 inhibits growth and migration of FLC. (A) Colony formation assays after treatment of control (n = 6) or miR-375 mimic (n = 6). Colony number (bottom left) and colony area (bottom right) were quantified. Data are presented from 2 independent experiments. (B) Scratch wound assays after treatment of control (n = 5) or miR-375 mimic (n = 7). Scale bar: 100 μm. Data are presented from 2 independent experiments. (C) RNA expression quantified by RNA-seq of the proliferation markers MKI67 and MCM2 after treatment of miR-375 mimic. Statistical significance was determined by the Wald test (DESeq2). (D) EdU incorporation assays after treatment of control (n = 3) or miR-375 mimic (n = 3). Data are representative of 2 independent experiments. *P < .05, **P < .01, ***P < .001 (Mann–Whitney U test, 1-sided, unless otherwise indicated), #P < .05 (Student 1-tailed t test, P ≥ .05 Mann–Whitney U test, 1-sided).