Table 2.
Antibodies | Catalog number | Company | Use | Dilution | Source |
---|---|---|---|---|---|
Primary | |||||
Epitope | |||||
E-cadherin | 334000 | Thermo Fisher | IF | 1:50 | Mouse |
Actin | A2066 | Sigma | WB | 1:10,000 | Rabbit |
p53 | sc-126 | Santa Cruz (Dallas, TX) | WB | 1:1000 | Mouse |
VSV | V4888 | Sigma | WB | 1:1000 | Rabbit |
p21 | 556431 | BD | WB | 1:1000 | Mouse |
NID1 | MAB2570 | R&D (Minneapolis, MN) | WB | 1:1000 | Mouse |
Secondary or conjugates | |||||
Anti-mouse HRP | W4021 | Promega | WB | 1:10,000 | Goat |
Anti-rabbit HRP | A0545 | Sigma | WB | 1:10,000 | Goat |
Alexa Flour 555, conjugated anti-mouse | A21422 | Thermo Fisher | IF | 1:500 | Goat |
HRP, horseradish peroxidase.