. 2018 Feb 21;9(1):48–55. doi: 10.15171/apb.2019.006

Table 1. Changes in Leydig cells key components involved in TES synthesis during aging .

Component	Affected components	Analysis technique	References
LH receptor density	Decreased mRNA level	DNA microarray	³⁹
StAR protein	Decreased mRNA level	Northern blotting Real-time quantitative PCR Western blotting	^40-42
	Decreased protein level
	Decreased activity
TSPO protein	Reduction in mRNA and protein levels	Northern blotting and Bradford method	⁴³
Mitochondrial P450_Scc	Decreased mRNA levels	Northern blotting	^40,41
Mitochondrial P450_Scc	Decreased protein levels	Western blotting	^40,41
3β-HSD, CYP17 & 17β-HSD3	Decreased mRNA levels	Northern blotting	³⁰
3β-HSD, CYP17 & 17β-HSD3	Decreased protein levels	Western blotting	³⁰

Abbreviations: StAR, steroidogenic acute regulatory protein; TSPO, translocator protein; 3β-HSD, 3β-hydroxysteroid dehydrogenase.