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. 2019 Feb 15;47(7):e40. doi: 10.1093/nar/gkz072

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — dCas9-α/ω mediated multi-directional transcriptional regulation. The position effects of dCas9-ω (A) and dCas9-α (B) on transcription regulation of P43-GFP. The relative transcription level represents the value of 2^−ΔΔCq that was measured by qRT-PCR. TSS means transcription start site of P43-GFP expression cassette. The distances of target sites to the TSS of P43-GFP expression cassette: G1, 88; G2, 78; G3, 42; G4, 24; G5, -28; G6, -77; G7, - 108; G8, -208; G9, -267; G10, -289; G11, -331; G12, -351; G13, -415.