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. 2019 Jan 30;47(7):3568–3579. doi: 10.1093/nar/gkz040

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — NgAgo-assisted gene editing independent of its potential enzymatic activity and guide DNA (gDNA). (A) Construction efficiency of isogenic lyi mutant in the P. multocida strain GX-PM based on NgAgo or the NgAgo mutants NgAgo-D663A and NgAgo- D663A-D738A, which are predicted to lack NgAgo potential catalytic activity. (B) Comparison of the construction efficiency of the isogenic lyi mutant in the P. multocida strain GX-PM based on NgAgo with or without gDNA. (C) Construction of isogenic lyi mutant in the P. multocida strain GX-PM with pSHK5(TS)-NgAgo-lyiLR, which was further inserted with other homologous sequences (opaL) or non-homologous sequences (gfp gene sequence).