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. 2019 Jan 31;47(7):3383–3394. doi: 10.1093/nar/gkz042

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Ada2b-isoform B is present in a small nuclear protein complex. Nuclear extracts (1.5 mg) from embryos expressing Ada2b-PBH₁F₂ and S2 cells expressing Ada2b-PBH₂F₂ were size-fractionated by Superose 6 gel filtration. The fractionation profile for Gcn5 and Ada2b was determined by Western blot. (A) Gcn5 and Ada2b consistently co-eluted in high molecular weight fractions 9–12, as expected for SAGA. They also co-eluted in smaller molecular weight fractions 17–19. (B) Endogenous Ada2b-PB consistently eluted in fractions 17–19 in extracts from wild-type embryos and S2 cells, indicating that the small complex is not a consequence of tagged Ada2b-PB overexpression. Fraction numbers and marker sizes are indicated, In: input. Asterisk (*): 62 kDa Ada2b-isoform B (Ada2b-PB). Arrowhead (<): 48 kDa Ada2b-isoform.