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. 2019 Jan 30;47(7):3365–3382. doi: 10.1093/nar/gkz041

Figure 5.

Figure 5.

SULF2 is a direct target of TRF2 and controls tumor angiogenesis. (A) Concentration of VEGF-A evaluated by ELISA in the CM of empty vector or SULF2 overexpressing HCT116 cells infected with scramble (shSCR), TRF2 (shTRF2) or SULF2 (shSULF2) targeting shRNAs. Results were normalized to cell number. (B, C) CMs described in A were assayed for their capability of inducing capillary structures in HUVEC cells. (B) Mean number of branching points calculated on five different fields and expressed as fold induction over the negative control. (C) Representative images showing tubular-like structures (5X magnification). (D) Concentration of VEGF-A evaluated in the CM obtained from HCT116 cells infected as indicated. CM were obtained from cells growth in serum-free medium for 24 h. Results were normalized to cell number. (E, F) CMs described in D were assayed for their angiogenic potential in vitro. (E) Mean number of branching points calculated as in B. (F) representative tubular-like structure images from E. The histograms show the mean ± SD of at least three independent experiments performed in triplicate (*P < 0.1, **P < 0.01, ***P < 0.001; Student's t-test).