Figure 1.

The mre11-S499P and rad50-A78T mutations phenocopy TEL1 deletion with respect to CPT hypersensitivity and telomere length. (A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT, MMS or phleomycin. (B) Telomere length. XhoI-cut genomic DNA from exponentially growing cells was subjected to Southern blot analysis using a poly(GT) telomere-specific probe. (C) Plasmid re-ligation assay. Cells were transformed with the same amounts of BamHI-linearized pRS316 plasmid DNA. Data are expressed as percentage of re-ligation relative to wild type that was set up at 100% after normalization to the corresponding transformation efficiency of the uncut plasmid. (D) Spore viability. Diploid cells homozygous for the indicated mutations were induced to enter in meiosis followed by tetrad dissection on YEPD plates. At least 40 tetrads for each strain have been analyzed. Mean values are represented with error bars denoting S.D. (n = 3).