Figure 1.

Twinkle BioID shows more than 8-fold enrichment for GRSF1 and MRPP1. Volcano plot showing statistically significant detected proteins (visualized in filled grey squares) in a Twinkle-bioID purification (n = 4) compared to control pull downs. Controls which are used include pull downs on the same cell line but without the addition of biotin to the medium (n = 3); pull downs performed on cell lines overexpressing a FLAG-tagged Twinkle protein (without a biotin ligase) (n = 2); and a pcDNA5 control cell line in which the cells were transfected with an empty plasmid and therefore that cell line is not overexpressing any protein (n = 1). These six controls all showed a very similar outcome and were therefore treated identically for the statistical analysis. The red square represents the position of the Twinkle protein (TWNK) in these pull downs and the blue squares represents a subset of well characterized RNA binding proteins as well as proteins involved in mtDNA maintenance which are statistically significantly enriched in Twinkle-bioID pull downs (the mitochondrial ribonuclease P protein1 or MRPP1 is indicated with its official symbol TRMT10C). The detailed results of the mass spectrometry results are given in Supplementary Table S1.