Figure 1.

Effects of tolvaptan and arginine vasopressin (AVP)/1‐deamino‐8‐d‐arginine vasopressin (dDAVP) on angiotensin II (AngII)‐induced aldosterone production in H295R cells. (a) Dose‐dependency of AngII on aldosterone production. Cells were stimulated with AngII at the indicated doses for 24 hr. (b) Effect of tolvaptan on AngII‐induced aldosterone production. Cells were pretreated with tolvaptan at the indicated doses for 30 min and then stimulated with 100 nmol·L⁻¹ AngII for 24 hr. (c) Effect of 1 μmol·L⁻¹ AVP or dDAVP on aldosterone production with and without 100 nmol·L⁻¹ AngII for 24 hr. Results are shown as mean ± SD (n = 5 separate experiments performed in duplicate). ^† P ˂ 0.05 versus Cont; *P ˂ 0.05 versus AngII