Figure 4.

Effects of angiotensin II (AngII) on aldosterone production, CYP11B2 protein expression, and systolic BP (SBP) in Sprague–Dawley rats. Rats were treated with AngII at the indicated doses. Results are shown as mean ± SD (n = 5 rats per group). (a) Dose‐dependency of effect of AngII on aldosterone production. ^† P ˂ 0.05 versus all other AngII‐treated groups. (b) CYP11B2 protein levels were analysed by Western blotting in whole adrenal tissue homogenates. Upper, representative immunoblots; lower, relative quantification of protein expression. ^† P ˂ 0.05 versus AngII 0 and 20 pmol·min⁻¹. (c) Dose dependency of effect of AngII on SBP elevation. ^† P ˂ 0.05 for AngII versus corresponding baseline