Figure 5.

Effects of tolvaptan (Tolv) on serum aldosterone and adrenal CYP11B2 protein levels as well as adrenal aldosterone production in Sprague–Dawley rats treated with angiotensin II (AngII). Rats were treated with tolvaptan in the presence and absence of 200 pmol·min⁻¹ AngII. Results are shown as mean ± SD (n = 8 rats per group). Effects of tolvaptan on AngII‐induced serum aldosterone (a) and CYP11B2 protein levels (b, upper, representative immunoblots; lower, relative quantification of protein expression) and adrenal aldosterone production (c). ^† P ˂ 0.05 versus Cont group; *P ˂ 0.05 versus AngII group; ^$ P ˂ 0.05 versus tolvaptan group