Figure 6.

Pretreatment with the selective MC₄ receptor antagonist HS024 abolished the effects of RO27‐3225 on neurobehavioural outcomes and ASK1/JNK/p‐38 MAPK/NLRP1/caspase‐1/IL‐1β signalling at 24 hr after ICH. (a–c) Forelimb placement, corner turn test, and modified Garcia test at 24 hr after ICH. (d) Representative western blot bands. Quantitative analyses of (e) phosphorylated ASK1, (f) phosphorylated JNK, (g) phosphorylated p38 MAPK, (h) NLRP1 inflammasome, (i) cleaved caspase‐1, and (j) IL‐1β at 24 hr after ICH. Data shown are individual values with mean ± SD; n = 6. *P < 0.05, significantly different from sham, ^# P < 0.05, significantly different from ICH + vehicle, ^$ P < 0.05, significantly different from ICH + RO27‐3225 + HS024; one‐way ANOVA, Tukey's test