Figure 3.
Reduced availability of SEMA3E in the gut increases the severity of experimental colitis. Colon were obtained from C57Bl/6 colitic mice treated with dextran sulfate sodium (DSS) 5% in the drinking water for 5 days or from non‐colitic mice (water). Sema3e +/+ mice received daily i.p. injection of plexin‐D1 soluble receptor (PLXD1; 10 μg·kg−1·day−1, R&D Systems) or 1% PBS with added human IgG Fc protein (Vehicle, Control) starting 1 day before DSS administration and lasted for 6 days. (a) Disease activity index and (b) macroscopic score. Colonic protein levels of pro‐inflammatory cytokines (c) IL‐6, (d) IL‐1β, and (e) TNF‐α. Data represent mean ± SEM (n = 6). *P < 0.05 and # refers to a significance when compared with control groups. One‐way ANOVA followed by multiple comparison test was applied