Figure 5.
The lack of SEMA3E aggravates acute dextran sulfate sodium (DSS)‐induced colitis, and treatment with SEMA3E improves disease severity. Sema3e +/+ and Sema3e −/− mice received 5% DSS in the drinking water to induce colitis, or drinking water, and were killed on day 5 post‐DSS. Mice received daily i.p. injection of SEMA3E recombinant protein (rec‐SEMA3E; 10 μg·kg−1·day−1; R&D Systems) or 1% PBS with added human IgG Fc protein (Vehicle, Control) for 6 days, starting 1 day before colitis induction. (a) Colonic protein levels of SEMA3E, (b) disease activity index, and (c) macroscopic score. Colonic expression of pro‐inflammatory cytokines (d) IL‐6, (e) IL‐1β, and (f) TNF‐α. Data represent mean ± SEM (n = 6, *P < 0.05). # refers to a significance when compared with control. One‐way ANOVA followed by multiple comparison test was applied