Figure 6.
The lack of SEMA3E aggravates acute dextran sulfate sodium (DSS)‐induced colitis and treatment with SEMA3E improves disease severity. Sema3e +/+ and Sema3e −/− mice received 5% DSS in the drinking water to induce colitis, or drinking water, and were killed on day 5 post‐DSS. Mice received daily i.p. injection of SEMA3E recombinant protein (rec‐SEMA3E; 10 μg·kg−1·day−1; R&D Systems) or 1% PBS with added human IgG Fc protein (Vehicle, Control) for 6 days, starting 1 day before colitis induction. (a) histological scores (Arbitrary Unit, AU) and (b) Haematoxylin–eosin staining of colonic tissues at 100× magnification power. Data represent mean ± SEM (n = 6, *P < 0.05). # refers to a significance when compared with control. One‐way ANOVA followed by multiple comparison test was applied