Figure 10.

In vivo SEMA3E treatment regulates in vitro interactions between splenic CD11c⁺ isolated from colitic mice and naïve CD4⁺CD25⁻ T‐cells. Sema3e ^+/+ and Sema3e ^−/− mice received 5% dextran sulfate sodium (DSS) in the drinking water to induce colitis and were killed on day 5 post‐DSS. Mice received daily i.p. injection of recombinant (rec‐) SEMA3E protein (10 μg·kg⁻¹·day⁻¹; R&D Systems) or 1% PBS with added human IgG Fc protein (Vehicle, Control) for 6 days, starting 1 day before colitis induction. Splenic CD11c⁺ were isolated from the different groups and co‐cultured with naive CD4⁺CD25⁻ T‐cells in the presence or absence of p19 or p35 monoclonal antibody (mAb) (10 μg·ml⁻¹) for 6 hr or with rec‐IL‐23 or rec‐IL‐12p70 (25 mg·ml⁻¹). (a, c) IFN‐γ and (b, d) IL‐17 levels in the medium were assessed. Data represent mean ± SEM (n = 6, *P < 0.05). # refers to a significance when compared with control. One‐way ANOVA followed by multiple comparison test was applied at significance level of 0.05