Primary structure, disulfide bridge alignment, and sequence homologies of CyrTx‐1a. (a) Primary structure of CyrTx‐1a. Asterisk denotes amidation. Disulfide bridging, as defined by homology, occurs according to the C1–C4, C2–C5, and C3–C6 pattern. (b) Consensus sequence of the NaSpTx family 1, adapted from Klint et al. (2012). (c) Comparison of amino acid sequences between CyrTx‐1a and the nine most similar analgesic toxins from NaSpTx family 1. Sequence alignment performed with Clustal Omega (version 1.2.4 from Emboss programs, EBlosum62 matrix for two pair alignment). The green shading highlights the percentage of identity (Jalview program according to EBlosum62 matrix)