Table 3.
Transcriptional response to ada-6 deletion by the genes involved in conidiation and vegetative cell wall development.
| Genes | Function annotation | RPKM Δada6-0 h | RPKM Δada6-12 h | RPKM Δada6-24 h | RPKM WT-0 h | RPKM WT-12 h | RPKM WT-24 h |
|---|---|---|---|---|---|---|---|
| Genes positively regulating conidiation | |||||||
| NCU04866 | Ada-6 | 0 | 0.045997 | 0.088329 | 7.139561 | 40.42227 | 19.05998 |
| NCU00116 | Aab-1, TF subunit | 41.0876 | 104.1344 | 177.4739 | 48.6871 | 114.6333 | 560.9975 |
| NCU00269 | Set-2 | 1.868055 | 2.594838 | 22.58605 | 2.667073 | 3.150217 | 6.208719 |
| NCU00929 | hypothetical protein | 9.3544 | 4.7866 | 7.945482 | 7.22975 | 4.6107335 | 25.61734 |
| NCU04813 | hypothetical protein | 13.3412 | 12.7783 | 9.8237 | 13.9730 | 15.9312 | 27.8958 |
| NCU05260 | Protein kinase | 7.46533 | 3.77964 | 5.44357 | 6.425309 | 4.244103 | 19.1833 |
| NCU07617 | Acon-3 | 0.186927 | 1.389872 | 3.91145 | 0.635499 | 8.159488 | 4.862642 |
| NCU08726 | fl, fluffy | 9.121856 | 17.91114 | 14.32059 | 17.76177 | 36.69432 | 45.58477 |
| Genes which deletion mutants with enhanced conidial production | |||||||
| NCU00355 | Cat-3 | 506.5213 | 574.7889 | 75.34231 | 237.5218 | 167.5816 | 19.71859 |
| Genes preferentially expressed during conidiation | |||||||
| NCU07324 | Con-13 | 0.300536 | 2.568501 | 2.56481 | 0.480817 | 7.380787 | 57.28341 |
| NCU07325 | Con-10 | 10.91534 | 72.60819 | 263.9326 | 7.64009 | 77.11508 | 984.083 |
| NCU08457 | Eas | 18.87683 | 270.4869 | 1248.747 | 82.90488 | 10027.32 | 17750.16 |
| NCU08769 | Con-6 | 3.702609 | 38.78643 | 69.66419 | 1.057798 | 72.8696 | 2500.538 |
| NCU09235 | Con-8 | 12.17338 | 203.7125 | 271.1714 | 26.04317 | 922.9603 | 1041.627 |
| NCU09357 | stage V sporulation protein K | 0.651945 | 13.99727 | 7.899063 | 3.809303 | 11.22863 | 23.29247 |
| Genes encoding vegetative cell wall protein | |||||||
| NCU05974 | Mwg1 | 224.3592 | 118.3476 | 191.3125 | 193.3397 | 63.22570 | 86.4393 |
| NCU07277 | Acw-8 | 569.5978 | 480.884 | 407.5197 | 517.1306 | 321.3095 | 64.3942 |
(1) WT, wild type; Δada6, ada-6 deletion mutant; -0 h, samples of N. crassa in vegetative growth stage; -12, samples of N. crassa after 12 h of conidiation induction; -24, samples of N. crassa after 24 h of conidiation induction. (2) Locus numbers and function were annotated according to the N. crassa genome assembly (http://fungidb.org/fungidb/). (3) RPKM, Reads Per Kilobase of exon model per Million mapped reads.