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. 2019 Jan 30;47(7):3536–3549. doi: 10.1093/nar/gkz035

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Lesion-specific repair of UV-induced DNA damage in living cells. (A and B) Mobility of (A) CPD-PL and (B) 6-4PP-PL as determined by FRAP analysis. Cells were non-irradiated (no UV), globally UV-C irradiated with 10 J/m² (UV), or globally UV-irradiated with 10 J/m² and photo-reactivated (UV + PR) for the indicated times by exposure to white light at 37°C. Cells were directly analyzed by FRAP after each treatment. “UV + 10 min mock” cells were UV-irradiated and mock photo-reactivated by shielding from white light during PR. (n ≥ 20 cells from two independent experiments) (C and D) GFP-XPC mobility was determined by FRAP in non-irradiated, globally UV-C irradiated (10 J/m²), or globally UV-C irradiated (10 J/m²) and photo-reactivated (10 min PR) in CPD-PL (C) or 6-4PP-PL (D) expressing cells. (E and F) Similarly, GFP-XPC mobility analysis was performed in DDB2 deficient cells (GFP-XPC DDB2–/–) (n ≥ 20 cells from 2 independent experiments).