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In vitro fluorescence labeling of poly(A) tail-modified reporter mRNAs. (A) Concept for poly(A) tail modification of mRNAs with 2′-N3-2′-dATP using yeast PAP followed by click reaction using DBCO-SRB. (B, C) Luciferase (GLuc, CLuc) and eGFP mRNAs were analyzed by PAGE (7.5% PAA gel, 10 W, 1 h, rt) and visualized using SRB fluorescence (λex = 532 nm) and SYBR™ Gold (Invitrogen) staining.
