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. 2019 Feb 7;47(7):3407–3421. doi: 10.1093/nar/gkz080

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — KAT2A and SIRT6 repress ADAM12 expression in normal cells. (A) Results of the chromatin screen: knockdown of KAT2A or SIRT6 causes the reactivation of ADAM12. Yellow dots represent ADAM12 normalized Nanostring counts in IMR90 transfected with targeting siRNAs; black dots for cells transfected with a non-targeting siRNA (siCtrl). (B) RT-qPCR representing relative expression of ADAM12, KAT2A and SIRT6 in IMR90 cells infected with siCtl (non-targeting siRNA), siKAT2A and/or siSIRT6. These siRNAs are independent from those used in the screen. Statistical analysis was performed with a one-way ANOVA followed by a Dunnett's test. (C) Western blot analysis showing efficient down regulation of KAT2A and SIRT6 after transfection of either the original pool of siRNA used in the screen (pool), or of individual siRNAs (ind.).