Figure 2.

Crystal packing of the CSB WHD–K48-Ub₂ complex. CSB WHD is colored green, except that the Ub-interacting α2 and C-terminal extremity are colored yellow and orange, respectively. The proximal and distal Ub moieties of K48-Ub₂ (Ub^prox and Ub^dist, respectively) are colored pink and cyan, respectively. The linkage between Gly76 of Ub^dist and Lys48 of Ub^prox is shown as sticks. The disordered linkage is indicated by a dotted line. (A) Four CSB WHD and two K48-Ub₂ molecules in the asymmetric unit. Four CSB WHD–Ub pairs (a–d) are encircled by dotted lines. (B) Structural comparison of the four CSB WHD–Ub complexes (a–d) in the asymmetric unit. The disordered regions are indicated by dotted lines.