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. 2019 Feb 11;47(7):3784–3794. doi: 10.1093/nar/gkz081

Figure 5.

Figure 5.

Assessment of the CSB WHD–Ub interaction in vivo. (A) Expression levels of CSBWT and mutant CSB proteins in the CS1ANSV-derived stable cell lines used in this study. The HA tag of each protein was detected by Western blotting using anti-HA tag antibody. Lamin B was also detected as a loading control by anti-lamin B antibody. (B) UV survival of WI38, CS1ANSV, and the CS1ANSV-derived stable cell lines expressing CSBWT or the indicated mutant CSB. The percentage of surviving cells is plotted against UV dose. Error bars indicate standard error from three independent experiments. (C) RNA synthesis recovery after UV irradiation in WI38, CS1ANSV, and the CS1ANSV-derived stable cell lines expressing CSBWT or the indicated mutant CSB. The ratio of the incorporation of [3H] uridine in 10 J m−2-irradiated cells to that of non-irradiated cells was considered to reflect the recovery of RNA synthesis after UV irradiation. Error bars indicate standard error from three independent experiments. Data were statistically analyzed by one-way analysis of variance (ANOVA) with Dunnett's post hoc tests (***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant).