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Metformin reduced the expression of HIF-1α in Caki-2 renal cancer cells. (A) Shows a Western blot analysis of HIF-1α in Caki-1 and Caki-2 cells incubated in the absence (control) or presence of metformin (2, 5, and 10 mM). Images are representative of three independent experiments per group. (B) Histograms represent pooled densitometry data normalized to β-actin expressed as the percentage of control. Data are presented as mean ± SEM.
