Table 3.

Effect of the dietary addition of aflatoxin B₁ (AFB₁) with or without Bacillus subtilis ANSB060 biodegradation product (BDP) on the concentration, excretion, and transfer rate of aflatoxin M₁ (AFM₁) in the milk of dairy cows at steady-state (days 4 to 8) (n = 8).

Item	Dietary Treatment 1		SEM	p-Value
	AF	AF + BDP
AFM1 concentration (ng L−1)	665 a	483 b	9	<0.01
AFM1 excretion 2 (μg d−1)	12.71 a	9.14 b	1.58	<0.01
Transfer rate 3 (%)	1.06 a	0.76 b	0.01	<0.01

^a,b Values in the same row with no common superscript differ significantly(p < 0.05). ¹ CON = basal TMR; AF = basal TMR + 63µg kg⁻¹ AFB₁, AF + BDP= basal TMR + 63µg kg⁻¹ AFB₁ + 2g kg⁻¹ BDP. ² AFM₁ excretion (μg d⁻¹) = concentration of AFM₁ in milk (ng L⁻¹) × milk yield (kg d⁻¹). ³ Transfer rate (%) = excretion of AFM₁ (ng d⁻¹)/AFB₁ consumption (ng d⁻¹) × 100.