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. 2019 Jan 30;9(1):19. doi: 10.3390/bios9010019

Figure 5.

Figure 5

Electropherograms show the purification of whole blood spiked with 10 ppm of gentamicin, amikacin, and tobramycin and derivatized with fluorescamine using a cross-chip (red trace) and the Double V- chip (black trace). The BGE in the V-sample waste channel was 10 mM phosphate buffer, pH 11.5, while in the separation channel, it was 100 mM phosphate buffer, pH 11.5, with 0.5% HPMC. Applied voltages for injection were −100, −700, −500, and +500 V for 60 s and for separation were −300, +220, +2000, and −400 V at reservoirs B, S, BW, and SW, respectively. For cross-channel, all channels were filled with 100 mM phosphate buffer, pH 11.5, with 0.5% HPMC. Applied voltages for injection were −100, −400, −300, and +400 V at B, S, BW, and SW reservoirs, respectively, and separation voltages were −200, +400, +1500, and +400 V at B, S, BW, and SW reservoirs. respectively.