Figure 2.

Dose-dependent effect of MSCs on clotting induction in human platelet-free plasma (PFP) using a thrombodynamics test. (A) Representative screenshots (at 72 s) of thrombodynamics videos showing spontaneous clot appearance in PFP after the addition of different doses of MSC. (B) Spontaneous clot formation time. (C) Representative screenshots at the end of assay (30 min) showing the area occupied by a clot in a cuvette. (D) Total clot formation time in the full volume of the cuvette. MSC aliquots of 5 × 10³, 1 × 10⁴, and 5 × 10⁴ cells and 10 μL of PBS instead of cells (“Without MSC” sample) were resuspended in human PFP obtained from 1 mL of freshly collected blood, then 120 µL of the mixture was sampled for analysis. Note, in the control PFP, spontaneous clot formation did not occur. Data reported as mean ± standard error of the mean (SEM) from at least five experiments. Experiments used the blood from five donors and each blood sample was incubated with MSCs obtained from three different umbilical cord samples. * p < 0.05, one-way ANOVA, followed by Tukey’s post hoc analysis.