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Modulation of MSC procoagulant activity by heparin pretreatment in vitro. (A) Representative diagram of thromboelastometry. (B) Clotting time and (C) clot formation time assayed by the NATEM test. MSCs were pretreated with 50 U heparin for 10 min, followed by washing three times in PBS. Further, MSCs were added to 1 mL of freshly obtained blood, reaching a final concentration of 5 × 104 cells per mL, then 300 µL of the suspension was sampled for analysis.
