Figure 6.
Array-based gene expression analysis of cancer stem-cell related genes after napabucasin treatment. Gene expression analysis of human cancer stem cell-related genes after 24 h of 1.25 µM napabucasin treatment using a RT2 Profiler Array. Data were normalized to five housekeeping genes (β–ACTIN, B2M, GAPDH, HPRT1, and RPLP0) according to the manufacturer’s suggestion (Qiagen Data Analysis Center) and related to untreated controls. Data are presented as mean value ± SEM related to untreated control cells of five individual biological replicates. For full gene names and RefSeq numbers see Supplementary Table S2. * indicates significant (p < 0.05) results.