. 2019 Mar 25;11(3):180. doi: 10.3390/toxins11030180

Table 1.

Identity and characteristics of the mycotoxins investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

R_t (min)	Mycotoxin Standard	MW	Parent Ion m/z (Precursor)	MS/MS Fragments	CE	R² (Neat Solvent)	R² (Sorghum Mix)	ARR (%)	LOD (µg/kg)	LOQ (µg/kg)
2.78	DON	296	297.10	231, 249	12	0.989	0.981	89	0.11	1.74
6.87	AFG₂	330	331.00	245, 313	32	0.999	0.992	82	0.01	0.04
7.12	AFG₁	328	329.00	243, 311	28	0.999	0.990	85	0.01	0.04
7.30	AFB₂	314	315.00	259, 287	31	0.999	0.989	91	0.59	1.98
7.48	AFB₁	312	313.00	241, 285	24	0.999	0.997	90	0.01	0.04
7.58	FB₁	721	722.40	352, 334	42	0.997	0.996	98	0.34	0.87
8.03	β-ZOL	322	323.00	277, 305	11	0.998	0.998	90	0.21	0.71
8.20	FB₂	705	706.10	336, 318	38	0.999	0.996	99	0.41	0.93
8.25	FB₃	705	706.30	336, 354	35	0.999	0.997	94	1.02	4.11
8.28	OTB	369	370.10	205, 324	14	0.998	0.991	94	0.06	0.19
8.38	α-ZOL	322	323.10	277, 305	9	0.999	0.993	87	0.65	2.17
8.53	T-2	466	467.20	245, 305	11	0.999	0.997	96	0.39	0.90
8.74	ZEA	318	319.10	185, 187	21	0.999	0.991	92	0.03	0.11
8.78	OTA	403	404.00	239, 221	38	0.999	0.993	86	0.04	0.14

ARR—apparent recovery rate; CE—collision energy; R_t—retention time; LOD—limit of detection; LOQ—limit of quantification; MW—molecular weight; m/z—mass-to-charge ratio; MS/MS—tandem mass spectrometry; R²—coefficient of regression; AFB₁—aflatoxin B₁; AFB₂—aflatoxin B₂; AFG₁—aflatoxin G₁; AFG₂—aflatoxin G₂; DON—deoxynivalenol; FB₁—fumonisin B₁; FB₂—fumonisin B₂; FB₃—fumonisin B₃; OTA—ochratoxin A; OTB—ochratoxin B; T-2—T-2 toxin; ZEA—zearalenone; α-ZOL—alpha-zearalenol; and β-ZOL—beta-zearalenol.