Table 2.
Serial dilution of purified D. palaestinae venom for injection into mice to determine its LD50.
Tube Number | Dose of Venom per Mouse (µg/0.5 mL) | Venom Concentration (µg/mL) | Volume from the Venom Stock Solution (100 µg/mL) | PBS (mL) |
---|---|---|---|---|
1 | 5 | 10 | 0.4 | 3.6 |
2 | 6.25 | 12.5 | 0.5 | 3.5 |
3 | 7.5 | 15 | 0.6 | 3.4 |
4 | 8.75 | 17.5 | 0.7 | 3.3 |
5 | 10 | 20 | 0.8 | 3.2 |
6 | 11.25 | 22.5 | 0.9 | 3.1 |
7 | 12.5 | 25 | 1 | 3 |
8 | 13.75 | 27.5 | 1.1 | 2.9 |
9 | 15 | 30 | 1.2 | 2.8 |
10 | 16.25 | 32.5 | 1.3 | 2.7 |
11 | 17.5 | 35 | 1.4 | 2.6 |
12 | 18.75 | 37.5 | 1.5 | 2.5 |
13 | 20 | 40 | 1.6 | 2.4 |
14 | 21.25 | 42.5 | 1.7 | 2.3 |
15 | 22.5 | 45 | 1.8 | 2.2 |
16 | 23.75 | 47.5 | 1.9 | 2.1 |