Skip to main content
. 2019 Mar 7;11(3):327. doi: 10.3390/cancers11030327

Figure 4.

Figure 4

Direct regulation of RACGAP1 by miR-204-5p in PDAC cells. (A) RACGAP1 mRNA expression in PDAC cell lines was evaluated by qRT-PCR at 72 h after transfection of miR-204-5p. GUSB expression was evaluated as the internal control. * p < 0.0001. (B) RACGAP1 protein expression in PDAC cell lines was evaluated by western blotting at 96 h after transfection of miR-204-5p. GAPDH expression was evaluated as the loading control. (C) miR-204-5p binding sites within the 3′-UTR of RACGAP1 mRNA. Dual luciferase reporter assays using vectors harboring either the wild-type RACGAP1 3′-UTR sequence containing the putative miR-204-5p binding site (nucleotide positions 851-857) or the 3′-UTR sequence with deletion of this binding site. Data represent the ratio of Renilla/firefly luciferase activities in PDAC cells transfected with miR-204-5p relative to that in mock-transfected control cells. * p < 0.0001; N.S.: no significant.