Figure 4.

Tracking of BKV-specific TCR clonotypes. BKV-specific TCR clonotypes were obtained from IFNγ producing T-cells after stimulation with BKV overlapping peptide pools. TCR clonotypes from whole blood CD4⁺ and CD8⁺ subsets were obtained at different stages of viral clearance. The overlap between the clonotypes of whole blood samples and clonotypes of the IFNγ producing T-cells were identified as BKV-specific T-cells in circulation. The abundance of these circulating BKV-specific T-cells were subsequently compared at distinct stages of viral clearance. Black bars indicate the frequency of clonotypes gained from the earlier to the later time point. Dark gray indicates the frequency of clonotypes found at both time points. Light gray indicates the frequency of clonotypes that has disappeared from the earlier time point to the later. (A) Relative change in BKV-specific clonotypes from the initiation of viral clearance (before) to the resolution of BKV infection (after). (B) Relative change in BKV-specific clonotypes from T-cells obtained during the clearance phase (mid) to the resolution of BKV infection (after). The bars indicate the frequency of clonotypes gained, lost, or sustained as the viral clearance progressed, that is, clonotypes that were present after viral clearance, but not before; clonotypes that were present at the beginning of the clearance phase but not after; and clonotypes present before and after viral clearance.