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. 2019 Feb 28;8(3):206. doi: 10.3390/cells8030206

Figure 3.

Figure 3

Schematic diagram illustrating the experimental design. (A) A small piece of the isocortex (parietal cortex) from Actin-eGFP mice at P0 was transplanted in replacement of the original entorhinal cortex of C57BL6/J in order to perform a mixed iso-hippocampal co-culture. After 6 DIV, biocytin tracer was injected in the isocortical region (asterisks). (B,C) Bright-field and fluorescence micrographs of Actin-eGFP brain showing the dissected area (asterisk). (D) After 7DIV, eGFP-positive isocortical axons were seen crossing the subiculum and entering the hippocampus. Dashed line indicates the boundary between the isocortex and hippocampus. (E,F) Sections from the middle and the bottom of the organotypic co-culture illustrating biocytin-labeled axons from the isocortical region. (G) A high-magnification of biocytin-labeled axons (arrows) innervating the SLM/ML boundary. (H,I) Electron micrographs showing asymmetric contacts (arrows) established between isocortical axons and apical dendrites of granular neurons. Abbreviations as in Figure 1 and Figure 2 and AT = axonal terminal; CWM = cortical white matter. Scale bars: D = 250 μm; E = 250 μm pertains to (F); G = 50 μm; H = 1 μm pertains to (I).