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. 2019 Mar 22;11(3):411. doi: 10.3390/cancers11030411

Figure 6.

Figure 6

Effects of various inhibitors on vacuole formation following honokiol, vacquinol-1, and MOMIPP treatment in U373 cells. Representative brightfield illustrations of U373 cells treated with or without the inhibitors are as follows: (a) pre-treated 1 h with bafilomycin A1 (100 nM) before honokiol (35 µM), MOMIPP (3 µM) or vacquinol-1 (5 µM) treatment, co-treatment with EHT1864 (25 µM) and the compound. Bafilomycin A1 inhibits the vacuoles induced by each compound but not ER-derived dark dense vacuoles induced by honokiol (white arrow). EHT1864 slightly inhibits vacuolization induced by honokiol and vacquinol-1 but not for vacuolization induced by MOMIPP. (b) Cells were co-treated with EIPA (10, 30 and 75 µM) and each compound. EIPA inhibits the vacuoles induced by each compound except the ER-derived dark dense vacuoles induced by honokiol (white arrow; similar than bafilomycin A1 effects). Each experiment has been conducted at least twice in triplicate.