Figure 8.

Illustration of the fluorescent characterization of the vacuoles using different fluorescent cell compartment trackers in U373 cells. Two kinds of vacuoles can be observed after treatment with honokiol: red arrows show acridine orange positive vacuoles possibly derived from macropinocytosis, and white arrows show ER-positive vacuoles. Some vacuoles induced by MOMIPP are red-positive with acridine orange (red arrow) but none to the ER-tracker. Vacquinol-1-induced vacuoles are red-positive (red arrow), but also green-positive stained by acridine orange. This experiment has been conducted three times in duplicate.