Figure 1.

The level of invadosome related proteins in HT-29 with Snail overexpression. Protein extracts from HT-29 stably transfected with pcDNA (control) or pcDNA/Snail vector (clone 8-SN8, clone 3-SN3) were harvested and analyzed by western blot using specific antibodies as described in methods section. (A) Grb2, Nck1/2, and cortactin level detected by western blot and (B) analyzed by densitometry and ImageJ software, performed out of 5 independent western blot experiments. The level of Snail expression in HT 29 clones, SN3 and SN8 have been shown previously [9]. ** p > 0.005.