Figure 8.

Effect of cathepsin B inhibition on invasion of HT-29 cells with Snail overexpression. Cells incubated for 24 h with cathepsin B inhibitor, CA-074ME (100 µM), or untreated once were seeded on Matrigel coated transwell inserts in the upper chamber in 0.1% BSA medium. Full medium in lower chamber served as chemoattractant for cell invasion. Cells were calculated after 6 h of incubation followed by hematoxylin/eosin staining. Number of control HT-29 cells that transmigrate into transwell membrane through 8 µM pores covered with Matrigel was set as 100%, next number of other cells was calculated and presented as % of control.