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. 2019 Mar 7;8(3):220. doi: 10.3390/cells8030220

Figure 7.

Figure 7

Effects of RNA interference on induction of Egr-1 protein by DIM-C-Pyr-4 and the specificity of protein knockdown. Effects of siRNAs on induction of Egr-1 protein by DIM-C-Pyr-4 in MCF-7 (A) and ZR-75 (B) cells. Cells were transfected with various siRNAs, treated with Me2SO or 5μM DIM-C-Pyr-4, and Egr-1 protein expression was determined by Western blot analysis of whole-cell lysates. Specificity of COUP-TFI, Sp1, Sp3, and Sp4 knockdown in MCF-7 (C) and ZR-75 (D) cells transfected with their corresponding siRNAs. Cells were transfected with iNT or various siRNAs, and COUP-TFI, Sp1, Sp3, and Sp4 protein expression was determined as described in the Materials and Methods section. α/β-Tubulin or histone H3 served as a protein loading control.