Table 3.
Kinetic parameters of α-glucosidase activities obtained from different samples of L. longipalpis females under different physiological conditions, using MUαGlu as substrate.
| MUαGlu |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Non-fed |
Sucrose-fed |
Blood-fed |
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| Sample | Vmax app (μU/Fleb) | Protein (μg/Fleb) | KM app (mM) | Vmax app (μU/Fleb) | Protein (μg/Fleb) | KM app (mM) | Vmax app (μU/Fleb) | Protein (μg/Fleb) | KM app (mM) |
| Crop | 90 ± 7 | 2.5 ± 0.5 | 2.1 ± 0.2 | 52 ± 2 | 2.7 ± 0.2 | 0.48 ± 0.06 | 16 ± 1 | 5.6 ± 0.7 | 0.9 ± 0.1 |
| Midgut content | 390 ± 20 | 2.76 ± 0.04 | 0.7 ± 0.1 | 266 ± 7 | 3.8 ± 0.3 | 0.37 ± 0.03 | 830 ± 10 | 258 ± 7 | 0.41 ± 0.02 |
| Midgut tissue | 3000 ± 200 | 10 ± 1 | 4.7 ± 0.4 | 1400 ± 100 | 6.2 ± 0.5 | 1.1 ± 0.1 | 1150 ± 40 | 337 ± 3 | 0.50 ± 0.05 |