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. 2019 Mar 19;11(3):386. doi: 10.3390/cancers11030386

Figure 4.

Figure 4

TCTP knockdown potentiates radiation-induced DNA damage in A549 cells. (a) After 48 h γ-radiation, γ-H2AX, H2AX, and TCTP expression level of A549 cells transfected with TCTP shRNA (shTCTP) and control shRNA (shCont) were measured using Western blot analysis (n = 4). The cropped blots are used in the figure and full-length blots are presented in Figure S8. The relative level of TCTP to β-actin and γ-H2AX to H2AX are indicated below each immunoblot image. (b) TCTP-depleted A549 cells were treated with 10 Gy of γ-radiation and comet assay was performed after 48 h. The olive tail moment was calculated using the Comet 5.5 software. The data represent the mean ± SEM. ** p < 0.01, *** p < 0.001 by one-way analysis of variance.