Figure 6.

TCTP knockdown enhances radiosensitivity in tumor xenograft mouse model. A549 cells were stably infected with lentiviral control shRNA (shCont) and shRNA vector-targeting TCTP (shTCTP) and introduced to the right thigh of 6-week-old, female, Balb/c nude mice. When the tumors of stably transfected cells reached 100 mm³, they were treated with γ-radiation (8 Gy). (a) The growth of the tumors was assessed by measuring tumor volume with digital caliper every other day for 25 days. The data represent the mean ± SEM. * p < 0.05, ** p < 0.01 vs. shCont. (b) Average tumor growth rate (mm³/days) is shown. The data represent the mean ± SEM (n = 6–8/group). * p < 0.05 (c) The immunoblots of tumors from shCont, shTCTP, shCont+IR, and shTCTP+IR groups using anti-p53, anti-TCTP, and anti-β-actin antibodies are shown. The cropped blots are used in the figure and full-length blots are presented in Figure S10. The relative level of proteins in comparison to β-actin are indicated below each immunoblot (n = 6). (d) Immunohistochemistry of the tumors with anti-Ki67 antibody and the percentage of Ki67-positive cells per field are shown. The scale bar indicates 50 μm (magnification: 200×). The data represent the mean ± SEM. * p < 0.05.