Figure 4.

The influence of dendrimers and glutamate on cerebellar granule cells’ (CGC) viability. (A) The cells were incubated for 30 min. in Locke medium in the presence of 0.2, 2 and 20 µM D24–D27 and viable cells were measured 24 h later. (B) cerebral granule cells were treated with 0.2 or 2 µM of D24, D25 or D27 together with 100 µM Glu for 30 min. in Locke 25 medium. Viable cells were counted after 24 h. D26 was excluded from this experiment based on data presented in Figure 4A where its high toxicity is shown. The results are presented as means ± E.S.Ds from 6 repetition performed using independently prepared cultures. * - Effect of the tested compound is significantly different from the 0.2% DMSO (solvent of dendrimers). # Effect of the tested compound is significantly different from that of the 100 µM Glu (p < 0.05, one-way analysis of variance (ANOVA)).