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. 2019 Mar 2;8(3):210. doi: 10.3390/cells8030210

Figure 6.

Figure 6

Analysis of resistance to apoptosis and anoikis in shRNA ROR1-knockdown and control clones. (A) PLC-shRNA ROR1-knockdown and control clones were treated with various concentration of Oxaliplatin for 24 h. PARP antibody was used to detect apoptosis. Protein band intensity of uncleaved PARP was measured by ImageJ to detect the ratio of surviving cells under drug treatment. (B) PLC-shRNA ROR1-knockdown and control clones were treated with various concentration of Doxorubicin for 24 h. PARP antibody was used to detect apoptosis. Protein band intensity of uncleaved PARP was measured by ImageJ to detect the ratio of surviving cells under drug treatment. β-actin antibody was used as a loading control. (C) shRNA ROR1-knockdown and control clones were seeded into ultra-low attachment plates and the ratio of apoptotic cells was calculated after 24 h via flow cytometry by using annexin V antibody. *** p < 0.05.