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. 2019 Mar 8;11(3):334. doi: 10.3390/cancers11030334

Figure 4.

Figure 4

Effects of early apoptosis inhibition on DDR and cell proliferation. (A) A549 cells were transfected with siRNA targeting sequences for both hnRNP A1 and A2 (siA1/A2) in the presence or absence of 20 mM of Z-VAD-FMK. Cells transfected with a non-target sequence (siNT) in the absence of Z-VAD-FMK served as a control. The transfected cells were cultured and monitored for cell proliferation using trypan blue staining. The data shown are pooled from 2 independent experiments; (B) After 72 h of transfection, the cells were fixed and immunostained for γH2AX (red) and TRF 2 (green), and the nuclei were counterstained with DAPI (blue). The foci of γH2AX that co-localized with TRF2 are indicated by marked squares. Cells treated with 50 μM of etoposide (Etop) for 12 h served as a negative control. The percentage of positive co-localization of γH2AX with TRF2 was determined from analysis of 30 γH2AX-positive nuclei from each experiment. The data shown in the bottom panel are from 2 independent experiments. * p < 0.05 versus siNT control. The scale bar equals 2 μm.