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. 2019 Mar 8;11(3):334. doi: 10.3390/cancers11030334

Figure 5.

Figure 5

Figure 5

Effects of suppressing hnRNP A1 and/or A2 on cell proliferation of normal fibroblasts and hTERT-immortalized normal fibroblasts. (A) Cell lysates from normal fibroblasts (HFF3, MRC5, and HFB) and hTERT-immortalized normal fibroblasts (hFF3-hTERT, MRC5-hTERT, and HFB-hTERT) were assayed for telomerase activity, as described in the materials and methods section. N represents a negative control with no cell extract, while P is a positive control with cell extract from telomerase-positive A549 cells; (B–D) Normal fibroblasts and hTERT-immortalized normal fibroblasts were transfected with siRNA targeting hnRNPA1 (siA1), hnRNPA2 (siA2), both hnRNP A1 and A2 (siA1/A2), or with a non-targeting sequence (siNT) for 72 h. Transfected cells were cultured in regular medium and monitored for cell proliferation using trypan blue staining. The results shown are pooled from 2 independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 versus siNT control.