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The steady-state kinetic parameters of CYP1A2 and the mutants oxidizing AFB1 into AFBO-GSH.
| Proteins | Km (μM) | Kcat (min−1) | Kcat/Km (min−1·mM−1) | Normalized Values (Kcat/Km) |
|---|---|---|---|---|
| WT | 29.86 ± 2.17 | 0.24 ± 0.007 | 8.0 | 1 |
| T124A | 6.79 ± 0.79 | 0.20 ± 0.004 | 29.5 | 3.69 |
| F125A | ND | ND | ND | ND |
| F226A | ND | ND | ND | ND |
| F260A | 457.35 ± 387.66 | 0.096 ± 0.067 | 0.2 | 0.025 |
Data are mean ± SEM based on at least three independent experiments. ND: not detected. WT: wide-type.
